Through-bond assignments
Sugar
1
H and
13
C resonances
Sequential sugar
1
H via the intervening
31
P.
Non-exchangeable base protons.
Intranucleotide sugar-to-base correlations.
Exchangeable imino and amino
1
H and
15
N base resonances.
Through-space assignments
Through-bond assignments
Sugar
1
H and
13
C resonances
Sequential sugar
1
H via the intervening
31
P.
Non-exchangeable base protons.
Intranucleotide sugar-to-base correlations.
Exchangeable imino and amino
1
H and
15
N base resonances.
Through-space assignments
Intranucleotide sugar-to-base correlations.
NMR experiments for labeled nucleic acids
Labeling strategies