Through-bond assignment of the non-exchangeable
base protons
Also see unlabeled nucleic acids
-
Directly-attached 1H-13C pairs are assigned from
1H-13C
2D Constant-time HSQC
experiment.
-
Usually 2D and 3D HCCH-COSY and
HCCH-TOCSY experiments are used for unambiguous
correlation of the Adenosine H2 and H8 resonances
(
94JACS2203
94JACS2205
99JMR345-137
) in 13C-labeled RNA and DNA.
-
Long-range optimized 1H-15N HSQC experiments is used
for assignment of nitrogen resonances
(
94JB117
).
-
2D and 3D HCN experiments
to correlate H6/H8 base protons with N1/N9 nitrogens.
-
2D (and 3D version) (H)N(C)-TOCSY-(C)H experiment based on HCP 1H-to-15N-to-13C
transfers followed by a 13C TOCSY step has been proposed for
correlating the amino nitrogen resonances to the H8 and H2 resonances in
adenine bases (
96JACS5316
).
-
3D-directed HCC-TOCSY-CCH-E.COSY experiment (
96JMRB160-112
),
Simplified spectra from directed TOCSY approach.