Through-bond assignment of the non-exchangeable
base protons
The non exchangeable base protons are:
| Proton |
Range 1H
chemical shift |
Range 13C
chemical shift |
|
C5
|
5.0-6.0
|
96.0-97.0
|
|
G8
|
7.4-7.8
|
135.0-137.5
|
|
T6
|
6.8-7.7
|
136.0-139.0
|
|
A8
|
7.85-8.3
|
138.5-140.5
|
|
C6
|
7.2-7.9
|
140.0-143.5
|
|
A2
|
6.8-7.8
|
152-153.5
|
|
U5
|
|
|
|
U6
|
|
|
|
TCH3
|
1.4-1.7
|
14.0-15.0
|
-
Directly-attached 1H-13C pairs are assigned from
standard 2D 1H-13C HMQC/
HSQC
experiments.
-
2D 1H-13C HMBC has also been applied to assign H2
and H8 protons in adenine residues (
96JB207-8
).