Directly-attached 1H-13C pairs are assigned from a
2D Constant-time HSQC
experiment.
Complete spin system assignment is performed from
2D and 3D HCCH-COSY
and HCCH-TOCSY
type experiments
(
92JACS9202
,
93JMB1141
,
94JACS4983
,
98JB25
, and
98JB559
).
Stereospecific H5' and H5" proton assignments can be achieved from long-range proton-carbon
coupling constants analysis extracted from multi-dimensional
HMQC-TOCSY
(
93JACS11002
and
94JACS5823
)
or HMQC-NOESY (
94JACS5823
)
experiments.
Analysis of cross-peak signal intensities
of C3'-H5 in 2D HMBC spectra can also be helpful
Stereospecific H2' and H2" proton assignments in deoxyribose can be achieved from
intraresidue di(1':2',2") NOE cross-peak analysis
or from the measurement of 3J1'2' and 3J1'2"
coupling constants.
3D HCN-CCH-COSY
(
00JMR147-145
) and 3D HCN-CCH-TOCSY
(
96JB251
,
99JB289
, and
00JMR147-145
) experiments allow the assignment of 1H and 13C resonances of ribose ring
by taking profit of the chemical dispersion of N1 of pyrimidine and N9 of purine
in a third independent dimension.
