Through-bond sequential assignment of
non-exchangeable 1H via the
intervening 31P
-
Heteronuclear
1H-31P correlations
2D 1H-31P
HMQC/HSQC
(referred as HELCO:
93JMRB81-102
,
00MRC288
,
01JB273-19
and 01JACS11306) optimized to 3,4JPH coupling constants.
Further assignments can be performed from a 3D
HELCO-TOCSY experiment(
00MRC288
) or from 2D 1H-31P Hetero-TOCSY
(
92JMR176-98
and
93JB577
)
experiments.
-
Relayed heteronuclear correlations from 2D or 3D hetero-TOCSY-TOCSY
(
94JMRB192-103
)
and hetero-TOCSY-NOESY
(
92JACS2727
,
93JB577
and
94BIO11460
)
experiments in which a conventional homonuclear TOCSY or NOESY building
block is inserted just priot to acquisition of a hetero-TOCSY pulse sequence.
-
3D HCPCH experiment
(
95JMRB117-109
)
via 2,3JCP and
1JCH coupling constants.
