Integrated Molecular Structure Education and Research Center (IMSERC) at Northwestern

All of the liquid NMR instruments, except au400, are equipped with autosamplers (SampleJet, SampleCasePlus, and SampleXpress).

Bio800

This newly installed Bruker Neo-X 800 MHz system w/ TCI-F cryoprobe is primarily used for macro-molecular NMR studies, including protein, DNA, RNA, and carbohydrates. Structure elucidation, natural product, and drug discovery can also benefit significantly from the extremely high sensitivity offered by the cryoprobe. In addtion, it has ultrahigh 19F sensitivity.

Features

TCI-F CryoProbe w/ Z-Gradient

Sensitivity: ¹ H=8600, ¹⁹ F=5500, ¹³ C=1550

VT range: -30° to +70°

Heteronuclear: No

Reservation: 1 hour to 168 hours per week

Other Features: Autotune, PFG at 65G/cm and SampleJet

A600

The Bruker Avance III 600 MHz system has two RF channels. It is used for extended variable temperature and hetero-nuclear NMR experiments in additon to regular proton and carbon ones.

Features

BBFO Smart Probe w/ Z-Gradient

Sensitivity: ¹ H=900, ¹³ C=360, ³¹ P=250, ¹⁹ F=960

VT range: -150° to +150°

Heteronuclear: Yes

Reservation: 1 hour to 48 hours per week

Other Features: autotune, PFG at 55G/cm, SampleCasePlus

Au400

Au400 is a 400 MHz Bruker Avance III HD Nanobay system equipped with SampleXpress autosampler. It is used for VT, reaction monitoring and hetero-nuclear NMR experiments.

Features

BBFO Smart probe w/ Z-Gradient

Sensitivity: ¹ H=500, ¹³ C=225, ³¹ P=150, ¹⁹ F=500

VT range: -150 to +150°

Heteronuclear: Yes

Reservation: 1 hour to 48 hours per week

Other Features: Autotune

Funding: National Science Foundation (CHE-1048773)

HFCN600

This Bruker Neo 600 MHz system w/ QCI-F cryoprobe is primarily used for macro-molecular NMR studies, including protein, DNA, RNA, and carbohydrates. Structure elucidation, natural product, and drug discovery can also benefit significantly from the extremely high sensitivity offered by the cryoprobe. In addtion, it has ultrahigh 19F sensitivity, the best around Chicago area.

Features

QCI (HFCN) CryoProbe w/ Z-Gradient

Sensitivity: ¹ H=4000, ¹⁹ F=6000, ¹³ C=800

VT range: -30° to +70°

Heteronuclear: No

Reservation: 1 hour to 168 hours per week

Other Features: Autotune, PFG at 55G/cm and SampleXpress

Ag500

The Bruker Avance III 500 MHz system is equipped with DCH CryoProbe. It is fully automated with a SampleCasePlus autosampler. It is located at B530 of Silverman Hall.

Features

CryoProbe 5mm DCH w/ Z-Gradient

Sensitivity: ¹ H=1800, ¹³ C=1400

VT range: 25°

Heteronuclear: No

Day Time Limit: 10 min per sample, up to 30 min during day time, no limit at night

Other Features: Autotune, Automation with SampleCasePlus

X500

The X500 is a Bruker Avance III HD system equipped with a BBO Prodigy CryoProbe, which improves the sensitivity by 2-3 times for the heteronuclei while still maintaining excellent sensitivity for proton. It is dedicated for high-throughput application. All the hetero-nuclear NMR experiments can benefit from the high sensitivity and convinience of the SampleCasePlus autosampler.

Features

BBO 5mm Prodigy CryoProbe w/ Z-Gradient

Sensitivity: ¹ H=1200, ¹³ C=700

VT range: 25°

Heteronuclear: Yes

Day Time Limit: 20 min per sample, up to 60 min during day time, no limit at night

Other Features: Autotune, SampleCasePlus, PFG at 55G/cm

Funding: Northwestern University

DNP600

The JEOL 600 MHz DNP system.

Features

Hg400

The Hg400 is a three channell 400 MHz Bruker Avance III HD system equipped with both liquid and solid-state probes. The liquid one is a BBFO Smart probe w/ gradient capable of doing most hetero-nuclear NMR experiments besides regular proton and carbon ones. It also has wide variable temperature capability. We have two solid-state probes on this system. The Bruker 4 mm HX probe is for most of route work. The Phoenix 1.6 mm HFX and 2.5 mm HXY probes can be used for wide variety of applications with spinning rate up to 40K Hz.

Features

BBFO Smart probe w/ Z-Gradient

Sensitivity: ¹ H=500, ¹³ C=225, ³¹ P=150, ¹⁹ F=500

VT range: -150° to +150°

Heteronuclear: Yes

Reservation: 1 hour to 48 hours per week

Other Features: Autotune

Funding: National Science Foundation (CHE-9871268) and International Institute of Nanotechnology

4 mm HX Bruker MAS SSNMR Probe

Sensitivity: ¹³C=225

VT range: -75° to +125°

Available nuclei frequency range: ¹H, ³¹P to ⁹¹Zr

Spinning rate: 5kHz to 14 kHz

Sample Volume/mass requirements: 106 ul (for liquid), 100 mg (for solid powder), If insert is used, then 25 ul for liquid and 40 mg for solid powder.

Reservation: No limit

Funding: National Science Foundation (CHE-9871268) and International Institute of Nanotechnology

2.5 mm HXY Phoenix MAS SSNMR Probe

Sensitivity: ¹³C=200

VT range: -125° to +125°

Available nuclei frequency range: Triple resonance model: H: ¹H to ¹⁹F, X: ³¹P to ¹³C, Y:²³Na to ⁵⁷Fe (below ¹⁵N need special low gamma box) Double resonance model: H: ¹H to ¹⁹F, X:³¹P to ⁵⁷Fe (below ¹⁵N need special low gamma box)

Spinning rate: 5 kHz to 25 kHz

Sample Volume/mass requirements: 20 ul (for liquid), 25 mg (for solid powder)

Reservation: No limit

Funding: Northwestern University

1.6 mm HFX Phoenix MAS SSNMR Probe

Sensitivity: ¹³C=72

VT range: -75° to +125°

Available nuclei frequency range: H: ¹H to ¹⁹F, X: ³¹P to ⁵⁷Fe (below ¹⁵N need special low gamma box)

Spinning rate: 8 kHz to 35 kHz

Sample Volume/mass requirements: 8 ul (for liquid), 12 mg (for solid powder).

Reservation: No limit

Funding: Northwestern University