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Experiment
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Conditions
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Correlation
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Related experiments
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2D
HSQC
2D
CT-HSQC
2D
HMQC
2D TROSY
2D
Reverse-INEPT
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Natural abundance
or X-labeling.
D2O or H2O.
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XH,X (X=13C
or 15N)
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Related pulse sequences for other measurements:
Relaxation rates
1H-X NOE effects
Proton exchange rates
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3D
HNCO
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13C,15N- labeling
H2O
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CO(i-1),N(i),NH(i)
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For small and medium-size proteins:
2D H(N)CO
2D H[NCO]
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3D
HN(CA)CO
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13C,15N- labeling
H2O
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CO(i-1),N(i),NH(i) via 2J(CA-N)
CO(i),N(i),NH(i) via 1J(CA-N)
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3D H(N)CACO
3D (HCA)CONH
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3D
HNCA
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13C,15N- labeling
H2O
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CA(i-1),N(i),NH(i) via 2J(CA-N)
CA(i),N(i),NH(i) via 1J(CA-N)
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2D H[NCA]
3D (HA)CANH
3D (HA)CA(CO)NH
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3D
HN(CO)CA
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13C,15N- labeling
H2O
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CA(i-1),N(i),NH(i)
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3D (HACA)CO,NH
2D HN(CO)(CA)
3D HN[COCA]
3D (HA)CA(CO)NH
3D H(N)COCA
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3D (HA)CANH
(also called as
(H)CANNH)
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13C,15N- labeling
H2O
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CA(i),N(i),NH(i) via 1J(CA-N)
CA(i),N(i+1),NH(i+1) via 2J(CA-N)
canh.GIF)
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Also see: 3D
HNCA
3D HA(CA)NH
3D HACA(N)H
3D HA[CAN]NH
3D HXYH
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3D
HA(CA)NH
(also H(CA)NH or
HA(CA)NNH)
3D HN(CA)HA
(also HN(CA)H)
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13C,15N- labeling
H2O
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NH(i),N(i),HA(i) via 1J(CA-N)
NH(i),N(i),HA(i-1) via 2J(CA-N)
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3D H(N)CAHA
3D (H)NCAHA
4D HNCAHA
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3D (HA)CA(CO)NH
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13C,15N- labeling
H2O
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CA(i-1),N(i),NH(i)
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Also see: 3D
HNCA
95JB189
,
97BIO14561
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3D HA(CACO)NH
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13C,15N- labeling
H2O
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HA(i-1),N(i),NH(i)
nh.GIF)
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3D HN(COCA)HA
3D [HACA](CO)NH
4D HN(CO)CAHA
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3D HCA(CO)N
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13C,15N- labeling
D2O
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HA(i-1),CA(i-1),N(i)
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2D HA(CA)N & 3D HACAN
2D HA(CACO)N
3D (CO)N(CO)CAH
4D HCACON
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3D
HCACO
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13C- labeling
D2O
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HA(i),CA(i),CO(i)
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3D HCACO-TOCSY
3D COCAH
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3D (H)N(COCA)NH
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13C,15N- labeling
H2O
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N(i),N(i-1),NH(i-1)
n(coca)nh.GIF)
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3D [HN](COCA)NH
4D HN(COCA)NH
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3D
(H)N(CA)NNH
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13C,15N- labeling
H2O
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N(i),N(i-1),NH(i-1) via 2J(CA-N) + 1J(CA-N)
N(i),N(i),NH(i) via 1J(CA-N) + 1J(CA-N)
N(i),N(i+1),NH(i+1) via 1J(CA-N)
+ 2J(CA-N)
nh.GIF)
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3D H(NCA)NNH
3D HN(CA)N
4D HN(CA)NH
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3D (H)NNH-TOCSY
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13C,15N- labeling
H2O
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N(i-1),N(i),NH(i)
N(i),N(i),NH(i)
N(i+1),N(i),NH(i)
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3D (H)CCH-TOCSY
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