Implementation on AVANCE spectrometers equipped with a third channel. Improved versions using pulsed field gradients (PFGs) are also available and, therefore, in such cases gradient technology is required.

The 3D HCA(CO)N experiment is applied on ¹³C,¹⁵N-labeled proteins in D₂O.

The 3D HCA(CO)N experiment has been described in 90JMR496-89 , 91JMR209-94 , and 92JB103 . Related versions have been also reported:

3D H(CA)CON experiment ( 01JMR246-153 )

2D HA(CA)N ( 95JB376 ) and 3D HACAN ( 00JB253 ) experiments.

2D HA(CACO)N experiment ( 95JB376 ).

3D (CO)N(CO)CAH experiment ( 97JMR149-125 )

4D HCACON experiment

See list of 3D triple-resonance NMR experiments for doubly-labeled proteins.