Implementation on AVANCE spectrometers equipped with a third channel. Improved versions using pulsed field gradients (PFGs) are also available and, therefore, in such cases gradient technology is required.

The 3D (H)N(COCA)NH experiment is applied on ¹³C,¹⁵N-labeled proteins in H₂O.

The 3D (H)N(COCA)NH experiment has been described in 96JMRB194-111 , 97JB94 , 05JB43-33) . A related 3D (H)N(CO-TOCSY)NH experiment has been reported for sequential resonance assignments in flexible polypeptides ( 00JB127 ) in that such connectivites are established in both directions. Related versions have been also reported:

3D H(NCOCA)NH experiment (05JB43-33).

3D [HN](COCA)NH experiment

3D (HNCO)CANH experiment (02JMR123-155)

4D HN(COCA)NH experiment

3D map displaying N(i), NH(i) and N(i-1) frequencies.

See list of 3D triple-resonance NMR experiments for doubly-labeled proteins.