Implementation on AVANCE spectrometers equipped with a third channel. Improved versions using pulsed field gradients (PFGs) are also available and, therefore, in such cases gradient technology is required.

The 3D HA(CACO)NH experiment is applied on ¹³C,¹⁵N-labeled proteins in H₂O.

The 3D HA(CACO)NH experiment has been described in 96JB98 , 96JMRB87-110 , and 97JACS9274 . Related versions have been also reported:

A related 3D HN(COCA)HA experiment uses a reverse coherence transfer pathway ( 92JB389 ) .

3D (HA)CA(CO)NH experiment

3D [HACA](CO)NH experiment ( 96JACS8146 and 94JMRB188-105 )

2D (HACACO)NH experiment to measure one-bond ¹HA-¹³CA dipolar couplings in ¹³C,¹⁵N-labeled proteins ( 99JMR281-140 )

2D (H)N(COCAHA)-TOCSY experiment for sequential inter- and intra-residue assignment ( 01MRC179 )

4D HN(CO)CAHA experiment

See list of 3D triple-resonance NMR experiments for doubly-labeled proteins.