Sample: Urea-15N, CH3OH-13C
A sample of 100mM 15N-labeled Urea and
13C-labeled 100mM CH3OH
in
DMSO-d6 is recommended to calibrate
high- and low-power 15N and 13C pulses to be applied
in inverse triple-resonance experiments. The 15N pulse
calibrations are performed on
the large doublet (88.5 Hz due to 1JNH) of the urea resonance
centered around 5.4ppm and the 13C pulse
calibrations
on the large doublet (139 Hz due to 1JCH)
of the methanol resonance centered around 3.17ppm.
The following tests can be performed:
List of spectra: