Sample: Urea/Methanol Solvent: DMSO Spectrometer: AVANCE 500 Probehead: Inverse Broadband with z-Gradients Experiment: Low-power 13C decouple pulse calibration Pulse program: decp90 ( Pulse Diagram) Experiment Time: 5 minutes Details: AVANCE Tutorial

This pulse calibration is performed using the paropt AU program. p3 is set to 80u and pl2 is varied from 13 to 20 dB using a 1 dB step. When using a small pl2 value (for instance, 13u), we observe antiphase signals at 3.2 ppm. pl2 is increased until the signals become zero (near 16dB) and this is the 90 degree 13C pulse from the decoupler at low-power level. These values are used for ¹³C GARP decoupling in all inverse applications. A similar procedure is followed to measure the High-power ¹³C pulse calibration from decoupler.

See list of spectra of Urea/Methanol sample