The TROSY approach has been successfully implemented in many
double- and triple-resonance
experiments (
98PNAS13585
,
99JMR439-139
and
00JACS7543
):
- Comparison of HSQC, TROSY, TROSY-Kay and CRINEPT for very large proteins at 900MHz
(
03JMR360-163
)
- Improved sensitivity by enhancement of the steady-state magnetization (ISIS-TROSY)
(
01JB99-21
)
- Constant-time 2D 1H-13C TROSY (
98JACS6394
and
99JMR439-139
) and 3D HCCH
experiments to be applied on aromatic 1H-13C spin
systems. Alternatively, TROSY also reduces transverse relaxation in 1H-13C moieties
of aromatic rings using cross-correlation between DD(13C-13C) and CSA(13C) of aromating ring
carbons,
as demonstrated for the 2D and
3D HCN experiment
(
01JACS658
).
- J-modulated TROSY to measure
J(HN-HA) coupling constants
(
01JMR60-151
)
- 2D [1H-13C]-[1H-13C]
TROSY for simultaneous detection of backbone NH, aromatic CH and
side-chain NH2 correlations
(
00JB195
)
-
3D 1H-15N NOESY-TROSY
and TOCSY-TROSY
(
99JB77
) experiments.
Suppression of diagonal peaks
(
99JMR499-140
and
99PNAS9607
) using the ZQ-TROSY
- 3D HSQC-NOESY-TROSY and
TROSY-NOESY-TROSY and
4D HSQC-NOESY-TROSY and
TROSY-NOESY-TROSY experiments.
- 3D TROSY-HNCO
and TROSY-HNCA
experiments. Application to the measurement of
scalar couplings across hydrogen bonds
(
98JACS8293
,
98PNAS14147
,
00JMR431-143
and
00JMR387-143
).
- 3D TROSY-HN(CO)CA,
TROSY-HN(CA)CO,
TROSY-HNCACB, and
TROSY-HN(CO)CACB
experiments
(
99JACS844
).
- 4D HNCOCA and
HNCACO experiments.
- Pulse sequences for protein
dynamics studies from
T1,
T2 and
H-NH NOE relaxation data
(
00JMR423-143
)
and for measuring chemical exchange
(
99JACS2331
,
99JB151-15
).
- Diagonal peak suppression
(
99JB377-14
,
99JMR499-140
,
99PNAS9607
, and
00JMR171-144
).
- Specifically for nucleic acids:
- Determination the interfaces in biomolecular complexes
using cross-saturation experiments
- The interference between conformational exchange-induced
time-dependent variations of chemical shifts can be produce interesting
TROSY-like improvements
(
01JB275-20
)
- Evaluation and optimization of coherence transfer in high molecular weight systems
(
02JMR123-155
)
- Methyl TROSY: HMQC spectra of protonated methyl groups in high molecular weigth, highly deuterated proteins exhibit large enhancements in sensitivity and resolution (
03JACS10420
,
03MRC843
,
04JB165-28
and
04JACS4921
).
