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3D HCN
DESCRIPTION
The 3D HCN experiment allows to obtain sugar-to-base correlations from the H1'(sugar) to N1/N9 (base) via C1'(sugar) (HsCsNb experiment)

or non-exchangeable base proton assignments from the H6/H8 (base) to N1/N9 (base) via C6/C8 (base) (HbCbNb experiment) in 13C,15N-labeled nucleic acids.

REQUIREMENTS
Implementation on AVANCE spectrometers equipped with a third channel and gradient technology.
VERSIONS
The original experiment was described in 93JB721 and 94JB129 .

Sensitivity-improved version using shaped pulses:

Improved sensitivity is achieved in MQC-based HCN experiments ( 97JACS7361 , 98JB373 , 98JMR119-130 , and 02JB9-22 )

and TROSY ( 00JB291 , 01JACS658 and 01JB367-21 ) experiments:

Modifications of the basic pulse scheme have been used to measure residual dipolar couplings in labeled nucleic acids ( 02JB9-22 , 05JB231-31).

Other approaches:

  • Several 2D H(C)N schemes have been compared ( 98JB373 ).
  • TROSY-based 2D H(C)N have been also proposed ( 01JACS658 and 01JB367-21 ).
  • Simultaneous acquisition of HCN and HCP experiments ( 96JB251 ).
  • 2D H(CN)C for sugar-to-base (2D Hs(CsNb)Cb) and base (2D Hb(CbNb)Cb) resonance assignments ( 04JB69-28 )
    • Related 2D and 3D sugar-to-base pulse sequences are: 2D Hs(CsNbCb)Hb
    2D (Hs)Cs(NbCb)Hb
    2D (Hb)Cb(NbCs)Hs
    2D Hs(CsNb)Hb
    3D HsCs(NbCb)Hb
    3D Hs(CsNb)CbHb
    3D (Hb)CbNb(Cs)Hs
    3D (Hs)CsNb(Cb)Hb
    EXPERIMENTAL DETAILS
    SPECTRA
    In the HCN spectra, all H/C pairs are sorted in a 2D spectrum as a function of 15N chemical shift in the third dimension.

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