Experiment
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Conditions
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Correlations
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Related Experiments
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2D amino-acid selective
1H-15N correlation
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13C,15
N-labeling
H2O
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HN(i),N(i) via 1J(CA-N) and
2J(CA-N) using the following pathways:
- 2D HN(CA) and HN(COCA) where CA=CH2
- 2D (CBCACO)NH and (CBCA)NH where CB=CH3
- 2D (CGCBCACO)NH and (CGCBCA)NH where CG=CH3
- selective version of 3
- 2D HN(COCACB) where CB=NH2
- 2D HN(COCACBCG) where CG=NH2
- Selective 2D (CDCGCBCACO)NH for Arg and Lys residues
- 2D HSQC spectra for (i)Trp, (ii)Phe,Tyr,His.
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Amino acids are selected based on the sidechain
spin topology
using a Multiple-quantum filter
(called as MUSIC):
- Glycine
- Alanine
- Thr,Ala,Val,Ile
- Thr,Ala
- Asn
- Asn,Gln
- Others ...
(
98JMR199-131
99JMR34-141
00JB331-17
01JMR61-148
01JMR186-153
01JB379-20
05JB115-31)
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3D HN(CA)HA-gly
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13C,15N-labeling
H2O
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HN(i),N(i),CA(i),HA(i) via 1J(CA-N)
HN(i-1),N(i-1),CA(i),HA(i) via 2JCA-N)
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Key: Two protons bound to alpha carbon
Experiment to selectively detect NH-CH2
spin systems.
Useful for:
Glycines
Residues following glycines
Side-chain NH-CH2(d)
in arginine residues.
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2D HN(COCA)
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13C,15N-labeling
H2O
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HN,N
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95JMRB206-109
For residues preceding glycine Two protons bound
to alpha carbon
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3D CDCA(NCO)CAHA
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13C,15N-labeling
H2O
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HD(i),CD(i)(t1),N(i),CO(i-1),CA(i-1)(t2),HA(i-1)
(t3)
via 1J(CD-N)
HA(i),CA(i)(t1),N(i),CO(i-1),CA(i-1)(t2),HA(i-1)
(t3) via 1J(CA-N)
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99JB381
Sequential assignments of prolines residues
with the HA chemical shifts of the preceding residue
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2D HACA(CO)(N)
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13C,15N-labeling
D2O
CENTER>
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HA(i),CA(i),CO(i),N(i-1)
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94JACS2215
For residues preceding proline
Key:
Lack of HN coupling
Also see
00JB331
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2D b-carbon edited (CBCACO)NH
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13C,15N-labeling
H2O
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NH,N
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For amino acids lacking a CB-CG coupling (serines,
cysteines, glycines, and alanines), or have j carbons well separated from
other aliphatic carbons (aromatic residues, asparagine
and aspartic acid).
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2D
(HCC)(CO)NH
3D (H)CC(CO)NH
3D H(CC)(CO)NH
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13C,15N-labeling
H2O
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NH,N
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For residues following methyl-containing amino acids
in fractionally perdeuterated
proteins
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2D and 3D HCN
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13C,15N-labeling
H2O |
1JCH + 1JCN
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96JMRB236-113
Selective observation of histidine and triptophan
H-C-N side chain spin
systems. Alternative to nJNH
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3D H2CCO |
13C- labeling
 
;
D2O |
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Identification od the acidic side-chain spin systems
in Asp and Glu
94BIO14858
2D H(C)CO2
97JMR274-124
Also 2D H[CCO2] experiment |