Spectral Editing by Residue Type  
See complete list of available MUSIC pulse programs.

Experiment
Conditions
Correlations
Related Experiments
2D amino-acid selective 1H-15N correlation
13C,15 N-labeling
H2O
HN(i),N(i) via 1J(CA-N) and 2J(CA-N) using the following pathways:
  1. 2D HN(CA) and HN(COCA) where CA=CH2
  2. 2D (CBCACO)NH and (CBCA)NH where CB=CH3
  3. 2D (CGCBCACO)NH and (CGCBCA)NH where CG=CH3
  4. selective version of 3
  5. 2D HN(COCACB) where CB=NH2
  6. 2D HN(COCACBCG) where CG=NH2
  7. Selective 2D (CDCGCBCACO)NH for Arg and Lys residues
  8. 2D HSQC spectra for (i)Trp, (ii)Phe,Tyr,His.
Amino acids are selected based on the sidechain spin topology using a Multiple-quantum filter (called as MUSIC):
  1. Glycine
  2. Alanine
  3. Thr,Ala,Val,Ile
  4. Thr,Ala
  5. Asn
  6. Asn,Gln
  7. Others ...
( 98JMR199-131
99JMR34-141
00JB331-17
01JMR61-148
01JMR186-153
01JB379-20
05JB115-31)
3D HN(CA)HA-gly
13C,15N-labeling
H2O
  • HN(i),N(i),CA(i),HA(i) via 1J(CA-N)
  • HN(i-1),N(i-1),CA(i),HA(i) via 2JCA-N)
  • Key: Two protons bound to alpha carbon

    Experiment to selectively detect NH-CH2 spin systems. Useful for:

  • Glycines
  • Residues following glycines
  • Side-chain NH-CH2(d) in arginine residues.
  • 2D HN(COCA)
    13C,15N-labeling
    H2O
  • HN,N
  • 95JMRB206-109
    For residues preceding glycine Two protons bound to alpha carbon
    3D CDCA(NCO)CAHA
    13C,15N-labeling
    H2O
  • HD(i),CD(i)(t1),N(i),CO(i-1),CA(i-1)(t2),HA(i-1) (t3)
    via 1J(CD-N)
  • HA(i),CA(i)(t1),N(i),CO(i-1),CA(i-1)(t2),HA(i-1) (t3)
    via 1J(CA-N)
  • 99JB381
    Sequential assignments of prolines residues with the HA chemical shifts of the preceding residue
    2D HACA(CO)(N)
    13C,15N-labeling
    D2O
  • HA(i),CA(i),CO(i),N(i-1)
  • 94JACS2215
    For residues preceding proline
    Key: Lack of HN coupling

    Also see 00JB331

    2D b-carbon edited
    (CBCACO)NH
    13C,15N-labeling
    H2O
    NH,N
    For amino acids lacking a CB-CG coupling (serines, cysteines, glycines, and alanines), or have j carbons well separated from other aliphatic carbons (aromatic residues, asparagine and aspartic acid).
    2D (HCC)(CO)NH
    3D (H)CC(CO)NH
    3D H(CC)(CO)NH
    13C,15N-labeling
    H2O
    NH,N
    For residues following methyl-containing amino acids in fractionally perdeuterated proteins
    2D and 3D HCN
    13C,15N-labeling
    H2O
    1JCH + 1JCN
    96JMRB236-113
    Selective observation of histidine and triptophan H-C-N side chain spin systems. Alternative to nJNH
    3D H2CCO
    13C- labeling
      ;
    D2O
    Identification od the acidic side-chain spin systems in Asp and Glu 
    94BIO14858
    2D H(C)CO2 
    97JMR274-124  
    Also 2D H[CCO2] experiment
    124'"+target+">"); 97JMR274-124  
    Also 2D H[CCO2] experiment