The 2D beta-carbon-edited (CBCACO)NH experiment provides sequential connectivities for amino acids lacking a CB-CG coupling (serines, cysteines, glycines, and alanines), or have j carbons well separated from other aliphatic carbons (aromatic residues, asparagine and aspartic acid).

Implementation on any AVANCE spectrometer equipped with an inverse probehead, a third channel and pulsed field gradients (PFGs). The experiment is applied on ¹³C,¹⁵N-labeled proteins in H₂O.

The original sequence ( 96JMRB107-110 and 96JMRB310-111 ) uses the same coherence transfer mechanism as the 3D CBCA(CO)NH experiment. Other versions have been reported:

A variant for deuterated proteins that uses the reverse pathway using the out-and-back approach (2D beta-carbon-edited HN(COCACB) experiment) ( 96JMRB95-112 )

2D (and 3D) TVI-(CBCACO)NH experiment to selectively detect sequential connectivites for threonines, valines and isoleucine residues ( 96JMRB304-110 ).

3D edited-CBCA(CO)NH experiment to trace out spin topologies based on the number of protons or carbons attached to CA and CB carbons ( 96JB345 ).