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The 2D w2-X-edited (filtered or selected) NOESY experiment affords a 2D NOESY spectrum in which 1H directly-attached to X nuclei are efficiently filtered or selected before the acquisition period. This experiment is widely used to study protein-ligand complexes, where the protein is usually 13C and/or 15N-labeled and the ligand is at natural abundance .REQUIREMENTS
Easy implementation on any AVANCE spectrometer equipped with an inverse probehead.VERSIONS
The basic sequence of the 2D w2-X-edited NOESY experiment is easily designed by replacing the fianl 90º 1H pulse of a conventional 2D NOESY pulse scheme by a X half-filter ( 92JACS2433 , 93JMRB322-102 , 97JACS6711 , and 99JB43 ).EXPERIMENTAL DETAILSRecently, a variant consisting of a 2D NOESY block followed by a HSQC-PEP block has been proposed to detect NOE between chemically equivalent protons in polycyclic aromatic hydrocarbons ( 00MRC311 ). Modified approaches have been reported using 13C/15N half filters (see w2-13C/15N-doubly edited NOESY experiment)
Other closely related experiments are:
The 2D w2-X-edited NOESY experiment can be recorded in routine/automation modes. The experiment is basically optimized as described for the 2D NOESY experiment. The X half-filter is optimized as described in a regular HMQC experiment and the phase cycle is selected in order to select or to filter the desired isotope.SPECTRATutorials: 2D inverse experiments Tutorials: 2D gradient-based inverse experiments
The 2D w2-X-edited NOESY spectrum shows a typical 2D NOESY map in which 1H directly attached to X are displayed in the F2 dimension.RELATED TOPICS
Analogs 3D NOESY-HMQC, 3D NOESY-HSQC, 3D HMQC-NOESY, and 3D HSQC-NOESY experiments can easily designed by incorporating a second variable evolution period in the HMQC/HSQC block as usual. On the other hand, a very similar pulse sequence is used for the 2D HMQC-NOESY and 2D HSQC-NOESY experiments by using a single variable evolution period in the HMQC/HSQC block .Related experiments:
2D Inverse experiments 2D Inverse gradient-enhanced experiments
