The 2D w₂-X/Y-edited (filtered or selected) NOESY experiment affords a 2D NOESY spectrum in which ¹H directly-attached to X and Y nuclei are efficiently filtered or selected before the acquisition period. This experiment is widely used to study protein-ligand complexes, where the protein is usually ¹³C and/or ¹⁵N-labeled and the ligand is at natural abundance .

Easy implementation on any AVANCE spectrometer equipped with an inverse probehead.

The experiment uses a doubly-edited ¹³C/¹⁵N half filters ( 99PROG93 ):

Other closely related experiments are:

2D w₂-X-edited NOESY experiment.

Tutorials: 2D inverse experiments

Tutorials: 2D gradient-based inverse experiments

The 2D w₂-X-edited NOESY spectrum shows a typical 2D NOESY map in which 1H directly attached to 12C/14N are displayed in the F2 dimension.

Analogs 3D NOESY-HMQC, 3D NOESY-HSQC, 3D HMQC-NOESY, and 3D HSQC-NOESY experiments can easily designed by incorporating a second variable evolution period in the HMQC/HSQC block as usual. On the other hand, a very similar pulse sequence is used for the 2D HMQC-NOESY and 2D HSQC-NOESY experiments by using a single variable evolution period in the HMQC/HSQC block .

Related experiments:

2D Inverse experiments

2D Inverse gradient-enhanced experiments