Basic Set-up

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Requirements Required 1H pulses Required 13C pulses Required band-selective 13C pulses Required 15N pulses Required 2H pulses Offsets and spectral widths Use of frequency lists Pre-optimized delays Parameter Calibration	Set-up Basic set-up of a 3D experiment Biomolecular sample preparation
Acquisition Acquisition parameter display Recording the two 2D planes	Processing Processing parameter display Phase corrections Processing the 3D spectrum Additional processing parameters Applying Linear prediction Removing residual water signal Streap Transform on the selected area