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Parameter Calibration for 3D experiments

f1 channel
NUC1=1H

  • 1H offset in Hertz (o1p)
  • 1H sweep width in ppm (3 sw)

  • 1H 90 (p1) and 180 (p2) hard pulse at high power level (pl1)
  • 1H decoupling pulse (pcpd1) at low power level (pl19)

  • 1H 90 TOCSY pulse (p6) at low power level (pl10)
  • General 1H trim pulse (p17)
  • 1H flip-back pulse shape (spnam1), power (sp1), and length (p11)
  • f2 channel
    NUC2=13C

  • 13C offset in Hertz (o2p)
  • 13C sweep width in ppm (1 sw and/or 2 sw)

  • 13C 90 (p3) and 180 (p4) hard pulse at high power level (pl2)
  • 13C decoupling pulse (pcpd2) at power level (pl12) using the scheme specified in cpdprg2.

  • 13C CC-TOCSY pulse (p9) at power level (pl15)
  • 13C 90deg shaped pulse name (spnam2), length (p13), and power (sp2)
  • 13C 90deg time-reversed shaped pulse name (spnam8) and power (sp8)
  • 13C 180deg shaped pulse name (spnam3), length (p14), and power (sp3)
  • 13C 180deg decoupling shaped pulse name (spnam3), length, and power (sp15)
  • f3 channel
    NUC3=15N

  • 15N offset in Hertz (o3p)
  • 15N sweep width in ppm (1 sw and/or 2 sw)

  • 15N 90 (p21) and 180 (p22) hard pulse at high power level (pl3)
  • 15N decoupling pulse (pcpd3) at power level (pl16) using the scheme specified in cpdprg3.