Protein NMR. Quantitative J Correlation

Quantitative J Correlation

In the quantitative J correlation approach ( 94METH79 ) , the strength of a long-range correlation in a nD spectrum is related in a simple manner to the intensity of the corresponding cross-peak taking as a reference an internal diagonal peak (method A) or an external reference spectrum (method B). Some applications of quantitative J correlation experiments have been reported:

Experiment	Original Reference	Dimensions	Coupling Constant	Method
3D HNHA		¹⁵N, ¹H, NH	J(HN-HA)	A
3D HAHB	94JACS9206	¹³C, ¹H, ¹H	J(HA-HB)	A
3D HNHB		¹⁵N, ¹H, NH	J(N-HA) J(N-HB)	A
3D HN(CO)HB	92JMR215		J(HB-CO)
3D HACAHB-COSY	95JACS5312 97JB263	¹³C, ¹H, ¹H	J(HA-HB)	A
3D LRCC	94JB787 92JACS6923	¹H, ¹³C, ¹H	nJ(CC)	B
3D LRCH spin-echo difference	93JB297 93JMRB228	¹³C, ¹H, ¹H	J(CH) to methyl carbons	B
3D HNCOCG		¹⁵N, ¹³C, ¹HN	J(CO-CG)	A
3D HNCG		¹⁵N, ¹³C, ¹HN	J(N-CG)	B
2D spin-echo difference CT-HSQC		¹⁵N, ¹H ¹³C, ¹H	J(N-CG) J(CO-CG) J(N-CG)	B
3D HN(CO)CO 3D (HN)CO(CO)NH		¹⁵N, ¹³CO,NH	J(CO-CO) J(CO-CG)	A
3D HN(CO)C		¹⁵N, ¹³C,NH	J(CO-CB) J(CO-CG)	B
2D H(N)CO		CO,NH	^3hJ_NCO through hydrogen bond