Protein NMR. J(CO-CG) Coupling Constants

³J_COCG

KARPLUS

97JACS6360

VALUES

In general < 4 Hz. Large values (greater than 3 Hz) are indicative of trans orientation, while small values (less than 1.5 Hz) are indicative of gauche orientation.

MEASUREMENT

E.COSY-type experiments
3D HCCH-COSY experiment ( 93JACS7878 , 94BIO5481 )
Quantitative J-correlation
1H-detected 1D ¹³C-¹³C long-range correlation ( 92JACS6923 ).
Simultaneous measurement of COCB and COCG from 3D HN(CO)C experiment.
2D ¹³C-{¹³CO} CT-HSQC and ¹³CO-{¹³CG} spin echo difference 1H-15N HSQC experiments using the spin-echo difference methodology.
3D HN(CO)CO experiment in Asn and Asp residues having side-chain CG carboxyl carbons.
3D HNCOCG experiment.

Measurement of 3J(CO-CG) in methyl groups using methyl-TROSY J-quantitative experiment ( 04JACS9827 )

Application of in the study of side-chain conformation in an unfolded protein ( 99JMB705 ).

RELATED TOPICS

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