TUTORIAL: 1D 1H-COUPLED 15N USING INEPT+

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1D ¹H-coupled¹⁵N spectrum (using INEPT+)

PRELIMINARY SET-UP

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Record a ¹H spectrum.

PARAMETER SET

a conventional ¹H-decoupled ¹⁵N spectrum with rpar N15INEPT all . Change the pulse program to ineptpnd

MODIFY SPECIFIC PARAMETERS

By default, the following parameters are set to: ns=32, ds=2, d1=5, sw=519 and td=32k. Change them if required. The interpulse delay d4 is optimized to 1/(4*¹J_NH) and d3 as a function of nitrogen multiplicity:

1/(6*¹J_NH) XH, XH2, XH3 positive
1/(4*¹J_NH) XH only
1/(3*¹J_NH) XH, XH3 positive, XH2 negative. By default, these value are set to 2.8 and 1.9 ms, respectively.

ACQUISITION

PROCESSING

PLOT

OBSERVATIONS

(au_zg)) (proc_1d).

OTHER RELATED VERSIONS

This same experiment can be recorded without refocusing and without decoupling. Only the pulse program might be changed: Non-refocused ¹H-coupled using INEPT (ineptnd)
¹H-decoupled using INEPT (ineptrd)