Applications for Targeted Quantitative Mass Spectrometry Studies
The Clinical Pharmacology Core, located within IMSERC, operates a sample preparation lab with biosafety hood, tissue homogenizer, centrifuge, 96 well plate solid phase extraction (SPE) system, SCIEX 6500 with Shimadzu UPLC, and Applied Biosystems API3000 with Agilent 1100 HPLC to enable targeted drug and metabolite studies in plasma and tissue. In addition, IMSERC houses multiple ion trap mass spectrometers, a Bruker Rapiflex MALDI system and Bruker Impact II Q-TOF for unknown metabolite and imaging experiments. CPC can partner with the High Throughput Laboratory for large (>500 sample) studies, the Developmental Therapeutic Core for animal studies, and NUCATS for clinical studies.
CPC supports determination of quantitation of drug stability and metabolic activity in using samples generated within PI labs. Contact a CPC staff member for assistance with setting up human liver microsomal / rat liver microsomal studies as well as CYP450 metabolic assays.
CPC can perform rapid PK analysis in animal models to perform bioavailability of novel compounds. Depending on the experimental setup, multicompartmental modeling can be performed at an additional charge.
CPC can perform tissue analysis to characterize drug penetration into tumor, brain, or other organs.