Before beginning a project or generating samples, CPC strongly suggests that a consultation be scheduled to identify:
- Experimental Goals
- Sample Collection Details
- Target Matrices and Analyte Concentration
- Number of Samples and Timeline
CPC staff can assist with grant proposal preparation to improve the chances of funding success. Typically data is collected on a fee-for-service basis and assistance with the grant proposal is provided free of charge when a CPC staff member will have dedicated effort on the project, if funded.
In order to complete assay development and sample analysis, the requester must provide:
- >= 10 mg of pure reference material, or a 1.0 mg/mL neat solution per compound.
- >= 10 mg of pure analog reference material, or a 1 mg/mL neat solution per compound for use as an internal standard. For GLP-like studies, stable isotope labeled analytes are strongly prefered. In cases where stable isotope labeled analyte cannot be obtained, CPC staff can suggest suitable alternative internal standards.
- >= 6 mL/ blank biological matrix or 5g tissue to be used for preparation of the calibration curve.
- >= 500 µL (plasma/blood/urine) and/or 60 mg (tissue) per sample to be analyzed. If smaller amounts of sample are provided, CPC staff will make their best effort to analyze in duplicate while reserving sufficient sample to rerun the sample preparation, if needed. This may result in an increased LLOQ or lower number of technical replicates.
Interpreting and Publishing Results
Data quality is independently confirmed by a second CPC staff member before release to the customer. At this time, the submitter may request additional modeling including muticompartmental kinetics modeling or generation of figure for publication. These activities are provided on a fee-for-service basis when no CPC member has effort applied to the project. In cases where this work leads to publication, CPC should be appropriately acknowledged and the appropriate staff member should be an author on the publication. Manuscript review prior to publication is provided at no cost.
Publications utilizing data collected in CPC should have the following language or similar statement in the acknowledgement section:
This work made use of the Clinical Pharmacology Core at Northwestern University, which has received support from the NIH (1S10OD012016-01 / 1S10RR019071-01A1), Soft and Hybrid Nanotechnology Experimental (SHyNE) Resource (NSF ECCS-1542205), the State of Illinois, and the International Institute for Nanotechnology (IIN).