• Record a conventional 1D ¹H presaturation spectrum. Note the frequency (o1) of the solvent resonance.

a solvent-suppressed 1D ¹H NOESY spectrum with rpar NOESYPR1D all (the pulse program noesypr1d

Presaturation experiments are recorded without sample spinning.

The power level and the duration of the presaturation are defined by  pl19 (start with 55 dB) and d1 (2 s), respectively).

Set mixing NOESY time (for instance, d8=300 ms).

o1 (on-resonance to the solvent resonance) may be finely adjusted in gs mode by minimizing the FID signal intensity.

It is advisable to store all acquisition and processing parameters (for instance, with the command wpar noesypr1d all) to be used later.

(au_watersc)) (proc_1d).

 See list of other solvent suppression methods.