NMR Experiments

Methods to measure dipolar couplings are based on the separate recording of a suitable NMR experiment in the absence (only scalar coupling) and presence (scalar and residual dipolar coupling) of an anisotropic medium

• ¹H-¹H dipolar couplings (D_HH)
• ¹H-¹⁵N dipolar couplings (¹D_NH)
• ¹HA-¹³CA dipolarcouplings (¹D_CA-HA)
• Other Heteronuclear Dipolar Couplings in labeled proteins:

  1D	2D	3D	NMR Experiment	Labeling	Reference
  N-H N-CO	H(N)-CO			13C,15N,2H	98JACS7385
  N-H HA-CA CA-CO N-CO	H(N)-CO				98JACS12334
  N-H CA-CO N-CO	H(N)-CO	H(N)-CA		13C,15N,2H	99JB333
  N-H HA-CA CA-CO N-CO N-CA	H(N)-CO H(N)-CA N-CA	H(N)-CA	3D spin-state-selective HNCO	13C,15N,2H	00JB43-17
  N-H CA-CO N-CO N-CA	H(N)-CO H(N)-CA N-CA	H(N)-CA	TROSY-based		00JB221
  N-CO			3D TROSY-HNCO		00JB101
  N-H CA-CO CA-HA			3D HNCO and 3D (HA)CA(CO)NH		01JB63
  CA-CB			2D and 3D TROSY-based HN(CO)CA		01JACS2858
  HA-CA	N-HA		HNCA-TROSY		03JB341-27
  HA-CA COCA	CO-HA		E.COSY-type HCACO Experiments		05JB321-31

• PMLG decoupling to eliminate the effects of RDCs evolution in liquid state applications. Implementation in INEPT and HMQC experiments ( 04JB443-30 )