1JNH

Range
Applications
Bibliographic References
  • Measurement of dipolar couplings
  • Measurement of through hydrogen-bond coupling constants in nucleic acids
  • 00JPCB2559-104
    96JMRB269-112
    98JB445-11
    98JB435-12
    98JMR373-131
    99JB335
    00JMR357-147
    00JMR184-143
    00JB361
    99JMR259-140

  • From w1-15N-coupled or w2-15N-coupled 2D HSQC experiments ( 96JACS6264) experiments of labeled molecules
  • Using the IPAP approach, in which two separate HSQC experiments generate in-phase and anti-phasecross-peaks. The sum and difference of such spectra affords spin-state separated cross-peaks that allow the measurement of coupling constant with high accuracy
  • Related spin-state selective excitation in the F2 dimension (2D HSQC-a/b experiment)
  • Accurate measurement of 1D(NH) using a J-quantitative CT-HSQC (HSQC-PEC) experiment (02JB195-23 )
  • 2D and 3D HNCO-type experiments, as for instance, 2D HN(CO)-IPAP experimentor a 3D TROSY-HNCO experiment.