Implementation on AVANCE spectrometers equipped with a third channel. Improved versions using pulsed field gradients (PFGs) are also available and, therefore, in such cases gradient technology is required.

The 3D (HA)CA(CO)NH experiment is applied on ¹³C,¹⁵N-labeled proteins in H₂O.

The original 3D (HA)CA(CO)NH experiment was proposed in 97JACS9576 and 97JB105 .

Related Experiments:

3D HA(CACO)NH

(HA)CANH experiment

3D HNCA

A related 3D HN(COCA)HA experiment uses a reverse coherence transfer pathway ( 92JB389 ) .

3D [HACA](CO)NH experiment ( 96JACS8146 and 94JMRB188-105 )

2D (HACACO)NH experiment to measure one-bond ¹HA-¹³CA dipolar couplings in ¹³C,¹⁵N-labeled proteins ( 99JMR281-140 )

See list of 3D triple-resonance NMR experiments for doubly-labeled proteins.