Dipolar Couplings   
Application of residual dipolar couplings (RDCs) in nucleic acids:
  • Structure determination and refinement of:
  • DNAs ( 00JACS9648, 01JACS3903 , 02JB1-24, 02JB317-24 and 03JB297-26)
  • RNA ( 00JACS11561 and 01JACS12135)
  • RNA-protein complex ( 99JB149-14)
  • Resonance assignment and simultaneous tertiary structure determination of RNA ( 02JMB637)
  • Periodicity in RDCs and nucleic acid structures ( 04JACS1938)
  • 1DCH measured for a 15mer DNA quelated to paramagnetic lanthanid ions ( 98JMR256-135) from a F2-coupled HSQC experiment.
  • Measurement of the H....H/H-N hydrogen bond length in double stranded DNA from 1J(NH) dipolar couplings ( 01JB361-19)
  • NMR Experiments
  • Homonuclear 1H-1H coupling constants:
  • from CT-COSY experiments ( 01JMR242-151 and 03PNAS11333)
  • H2'-C2 and H3'-C3 RDCs in RNA molecules from 3D IPAP-based HCCH-COSY experiments.
  • A suite of S3E schemes has been proposed to measure small scalar and dipolar 1DCC, 1DCN, 2DCH, 2DNH in 13C,15N labeled purine and pyrimidine bases ( 01JB153-21).
  • MQ-HCN-based pulse sequences to measure a series of one-bond and two-bonds dipolar couplings in labeled DNA ( 02JB9-22). Related J-quantitative experiments have been also reported to measure 15N-13C RDCs in nucleic acids ( 05JB231-31).
  • Five RDCs form a single 3D H1C1C2 experiment ( 04JACS66-126)
  • Mesaurement of D(CH) and D(HH) for methine-methylene pairs using spin-edited E.COSY type HSQC experiments ( 05JB201-31 )
  • 1DCH, 1DNH and DHH of a DNA dodecamer in aqueous crystalline medium containing 5% bicelles ( 00JACS6190) and DHH and DPH in liquid crystalline Pf1 medium ( 03JB297-26).
  • 1DCH in RNA-antibiotic complexes ( 00JACS7853).
  • 1D(CH), 1D(CC) and 2D(CH) using 3D HCC TROSY experiments displaying E.COSY multiplet patterns ( 04JB287-30).
  • Determination of helical parameters from one-bond and two-bond 1H-13C, 1H-15N, 13C-13C, and 13C-15N residual dipolar couplings using the METHADON method ( 00JACS10454)
  • Determination of backbone angles in 13C-labeled RNA using CH dipolar couplings ( 00JACS12728)
  • Through-space DPH dipolar couplings between 31P and 1H in RNA ( 01JACS3395) using the RADAR-TOCSY experiment.
  • Measurement of 1H-19F dipolar couplings in fluorolabeled RNA ( 01JB39-20 ) from X-filtered E.COSY spectra.
  • 31P Chemical shift anisotropy observed in liquid crystals
  • Temperature dependence of D(HH) in adenosine in charged phospholipid bicelles ( 02MRC225 )
    • Also see Dipolar Couplings in Proteins
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