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The 2D w1-X/Y-edited (filtered or selected) NOESY experiment affords a 2D NOESY spectrum in which 1H directly-attached to X nuclei are efficiently filtered before evolution during the variable t1 period. This experiment is widely used to study protein-ligand complexes, where the protein is usually 13C and/or 15N-labeled and the ligand is at natural abundance .REQUIREMENTS
Easy implementation on any AVANCE spectrometer equipped with an inverse probehead.VERSIONS
EXPERIMENTAL DETAILSModified approaches have been reported using 13C/15N half filters ( 99PROG93 ):
Other closely related experiments are:
2D w1-X-edited NOESY experiment.
SPECTRATutorials: 2D inverse experiments Tutorials: 2D gradient-based inverse experiments
RELATED TOPICS
Analogs 3D NOESY-HMQC, 3D NOESY-HSQC, 3D HMQC-NOESY, and 3D HSQC-NOESY experiments can easily designed by incorporating a second variable evolution period in the HMQC/HSQC block as usual. On the other hand, a very similar pulse sequence is used for the 2D HMQC-NOESY and 2D HSQC-NOESY experiments by using a single variable evolution period in the HMQC/HSQC block .Related experiments:
2D Inverse experiments 2D Inverse gradient-enhanced experiments