Sample: Strychnine Solvent: CDCl3 Spectrometer: AVANCE 400 Probehead: Inverse Broadband with z-Gradients Experiment: Conventional 13C spectrum Pulse program: zgpg30 ( Pulse Diagram) Experiment Time: 5 minutes Details: AVANCE Tutorial

The ¹³C spectrum of strychnine shows the following features:

12 aliphatic signals resonating between 25 and 78 ppm, demonstrating the possible presence of heteroatoms or ring systems.

9 signals resonating in the region 116-170 ppm. Five of them correspond to methine protons, as shown by their intensity and as demonstrated in the DEPT spectra. The other four correspond to quaternary carbons. In particular, the signal at 169.1 ppm is typical of a carbonyl carbon belonging to an ester or amide functional group

Unambiguous assignment of all protonated carbons is achieved from the 2D ¹H-¹³C HSQC spectra, and the quaternary carbons can be ellucidated using the Magnitude-mode ge-2D ¹H-¹³C HMBC experiment. Multiplicity information can be extracted from the DEPT135 and DEPT90 spectra or from ge-2D ¹H-¹³C HSQC multiplicity-edited experiment.

See list of spectra of strychnine