• Record a conventional ¹H spectrum. Note the frequency (o1) of the solvent resonance.

a ¹H WATERGATE spectrum with rpar ZGGPWG all (the pulse program zggpwg

Solvent suppression experiments are recorded without sample spinning.

Set o1 on-resonance to the solvent resonance to be minimized (o1 may be finely adjusted in gs mode). Increase sw as required.

The basic gradient parameters are:

p16 is the duration of the gradient (1 ms)
d16 is the recovery delay after the gradient (100u)
gpz1=20
gpnam1=SINE.100

In this version of the watergate scheme, solvent suppression is achieved using water-selective 90 pulses. Parameters defining such pulses are:

sp1is the power level for these pulses.
p11is the 90 degree shaped pulse at sp1 power level (see Selective 90º 1H observe pulse calibration). It can be finely adjusted in gs mode to improve the suppression level.
phcor2 if the phase difference between hard and soft pulses.

Minimum number of scans ns=8 and ds=4.

Watergate scheme is easily implemented in many multidimensional experiments. Thus, the experimental conditions found for the standard 1D version can be used in more sophisticated 2D, 3D, and 4D experiments.

List of Related Experiments:

Solvent suppression methods
Gradient-enhanced 2D experiments in H₂O