• Record a conventional ¹H spectrum. Note the frequency (o1) of the solvent resonance.

a ¹H WATERGATE spectrum with rpar P3919GP all (the pulse program p3919gp

Solvent suppression experiments are recorded without sample spinning.

Set o1 on-resonance to the solvent resonance to be minimized (o1 may be finely adjusted in gs mode). Increase sw as required.

The basic gradient parameters are:

p16 is the duration of the gradient (1 ms)
d16 is the recovery delay after the gradient (100u)
gpz1=gpz2=20
gpnam1=gpnam2=SINE.100

In this version of the watergate scheme, solvent suppression is achieved using a 3-9-19 pulse train. Parameters defining such train are:

pl18 is the power level for these pulses
p27is the 90 degree pulse at pl18 (see 90º 1H observe pulse calibration)
2*d19 is the interpulse delay (start with d9=150 usec). Check for the first sidebands appearing to 1/(2*d19).

p0 (in principle p0=p27) is the last 90º pulse of the 3-9-19 pulse train. It can also be finely adjusted in gs mode to improve the suppression level.

. Minimum number of scans ns=8 and ds=4.

Watergate scheme is easily implemented in many multidimensional experiments. Thus, the experimental conditions found for the standard 1D version can be used in more sophisticated 2D, 3D, and 4D experiments.

List of Related Experiments:

Solvent suppression methods
Gradient-enhanced 2D experiments in H₂O