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2D 1H DOSY experiment

PRELIMINARY SET-UP PARAMETER SET
  • From the above file, create a new dataset using edc or iexpno and change parmode to 2D.
  • Enter the corresponding pulprog as a function of the chosen experiment:
  •  
    Experiment
    pulprog
    Stimulated Echo experiment (STE)
    stegp1s
    LED experiment
    ledgp2s
    LED experiment using bipolar gradients (LEDbp)
    ledbpgp2s
    Double-Stimulated Echo Experiment (DSTE)
    dstegp3s
    MODIFY SPECIFIC PARAMETERS
    The following parameters are used in the pulse programs and are evaluated for the processing:
  • d20 the Stejskal-Tanner diffusion delay
  • d21 eddy-current delay
  • p30 gradient duration
  • d16 gradient recovery delay
  • Set the following gradient parameters:
    Gradient ratios: gpz6 100(variable) , gpz7 -17.13, gpz8 -13.17, and gpz9 -15.37,
    Gradient Lengths: p30 1 ms and p19 1.1 ms.
    Recovery delay: d16 100u.
    The diffusion and the eddy-current times are d20 and d21. Standard value can be 200 and 50 ms, respectively.

    ACQUISITION

    Define the number of proton spectra to be acquired (for instance, set td1 to 32) and call the AU program dosy to create the gradient ramp function:

    Enter first gradieny amplitude: 2
    Enter final gradieny amplitude: 95
    Enter number of points: 32
    ram type (l/q): l
    and finally, Do you want to start acquisition? and select OK or Cancel.
    PROCESSING
    Use the eddosy window to set processing parameters and process the data with the xf2 and dosy2d commands. See Processing 2D DOSY experiments for more details.

    Otherwhise, all 1D spectra can be visualized using the AU splitser program which splits the ser file into single fids, starting with the expno which follows the ser file. Then, all these 1D spectra can be processed in the same way using the multiefp program and analyzed using multi_integ . An automated analysis can also be performed using the setdiffparm AU program.

    OBSERVATIONS
    It is advisable to store acquisition and processing parameters (for instance, with the command wpar dosy2d all) to be used later.
    OTHER RELATED VERSIONS
    A quick and semi-automated set-up procedure can be followed if a parameter set has been previously stored in the disk. Such procedure can also be executed using an AU  or a macro program.