2D 1H-15N Inverse & Hybrid

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Important Note: All described pulse programs use the f3 decoupler channel for the ¹⁵N nucleus. The same results should be obtained from analog pulse programs using the f2 channel (see, for instance, gradient-based 2D 1H-13C inverse & hybrid experiments. On the other hand, they could also be applied on other nuclei, for instance, if a triple-channel broadband probehead is available. In such case, pulses, delays and gradient strengths should be set accordingly.

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ge-2D ¹H-¹⁵N HMQC-TOCSY experiment

• Phase sensitive ge-2D ¹H-¹⁵N HMQC-TOCSY with DIPSI-2 using echo-antiecho (hmqcdietf3gp)
• Phase sensitive ge-2D ¹H-¹⁵N HMQC-TOCSY with DIPSI-2 using PEP (hmqcdietf3gpsi)
• Phase sensitive ge-2D ¹H-¹⁵N HMQC-TOCSY with DIPSI-2 using PEP and shorther overall timing( hmqcdietf3gpsi.2)

ge-2D ¹H-¹⁵N HSQC-TOCSY experiment

• Phase sensitive ge-2D ¹H-¹⁵N HSQC-TOCSY with MLEV using echo-antiecho (hsqcetf3gpml)
• Phase sensitive ge-2D ¹H-¹⁵N HSQC-TOCSY with DIPSI-2 using PEP (hsqcdietf3gpsi | HSQCDIETF3GPSI)

ge-2D ¹H-¹⁵N HMQC-NOESY experiment

• Phase-sensitive ge-2D ¹H-¹⁵N HMQC-NOESY using echo-antiecho (hmqcetf3gpno)

ge-2D ¹H-¹⁵N HMQC-ROESY experiment

• Phase-sensitive ge-2D ¹H-¹⁵N HMQC-ROESY using echo-antiecho (hmqcetf3gpro)
• Phase-sensitive ge-2D ¹H-¹⁵N HMQC-T-ROESY using echo-antiecho (hmqcetf3gpro.2)

ge-2D ¹H-¹⁵N HSQC-NOESY experiment

• Phase-sensitive ge-2D ¹H-¹⁵N HSQC-NOESY using echo-antiecho (hsqcetf3gpno | HSQCETF3GPNO)

ge-2D ¹H-¹⁵N HSQC-ROESY experiment

• Phase-sensitive ge-2D ¹H-¹⁵N HSQC-ROESY using echo-antiecho (hsqcetf3gpro)
• Phase-sensitive ge-2D ¹H-¹⁵N HSQC-T-ROESY using echo-antiecho (hsqcetf3gpro.2)