• Record a conventional ¹H-decoupled ¹³C spectrum.

From the standard ¹³C spectrum file, create a new dataset with and change the pulse program to pulprog ineptnd. This results in a ¹H-coupled ¹³C spectrum in which resonances shown anti-phase pattern with respect ¹J_CH. 

.

See  90 13C observe pulse and 90 1H decouple pulse calibrations).

The interpulse delay (d4) is optimized to 1/[4*¹J_CH]. A typical value is d4=1.78m (for ¹J_CH=140Hz).

Set ns as a function of the sample concentration.

(au_zg)) (proc_1d).

Improved versions of the INEPT experiment are:

• INEPT+ experiment. From the basic ineptnd parameters, change the pulse program to pulprog ineptpnd, optimize d3 to 1/[4*J_CH] (a typical value is d3=2.68m (for ¹J_CH=140Hz)) and start the acquisition under the same conditions. The result is a multiplicity-edited ¹H-coupled ¹³C spectrum. All acquisition and processing parameters can be stored by typing wpar ineptpnd all.
• Refocused INEPT experiment. From the basic ineptnd parameters, change the pulse program to pulprog ineptrd, optimize d3 to 1/[4*J_CH] (a typical value is d3=2.68m (for ¹J_CH=140Hz)) and start the acquisition under the same conditions. Similarly as described for DEPT experiments , the d3 delay  can be optimized as a function of carbon multiplicity:

    1/(6J_CH)  CH, CH₂, CH₃ positive
    1/(4J_CH)  CH only
    1/(3J_CH)  CH, CH₃ positive, CH₂ negative.
The result is a multiplicity-edited ¹H-decoupled ¹³C spectrum. All acquisition and processing parameters can be stored by typing wpar ineptrd all.