• Record a conventional ¹³C spectrum.

From the reference ¹³C spectrum create a new dataset with and change the pulse program to pulprog inad1d. All other parameters as in the ¹³C spectrum. 

It is very important to use a long recycle delay, usually d1=5*T₁(¹³C) (for protonated carbons, start with d1=3 s).

The interpulse delay (d4) is optimized to 1/[4*J_CC] (d4=7.6 msec, calculated from ¹J_CC=33 Hz).

Set ns as a function of sample concentration (minimum phase cycle with ns=16 and ds=16). Due to its low sensitivity, set ns to a large value (ns=1024)

Adjust the antiphase pattern of the ¹³C resonances.

Several versions are also available:

1D INADEQUATE experiment using composite pulses (pulprog inadcp1d).
1D refocused INADEQUATE experiment (pulprog inadrd1d).