• Record a conventional 1H-decoupled 13C spectrum.

a DEPT-90 spectrum with rpar C13DEPT90 all (the pulse program dept90

See  90º 13C observe pulse and 90º 1H decouple pulse calibrations.

By default, the following parameters are set to: ns=128, ds=2, d1=2, d2=3.57ms, sw=331.2 and td=64k. Change them if required.

Optimize the values of o1 and sw from the conventional 13C spectrum if required. o2 must be set to the center of the proton spectrum.

(au_zg)) (proc_1d).

In automation applications the signal-to-noise ratio can be automatically optimized changing the acquisition AU program to AUNM=au_deptsino.

For instance, to record a DEPT-45 spectrum, read the predefined parameter set of DEPT-90 experiment (rpar dept90 all), change the pulse program (pulprog=dept45) and start the acquisition (check for the number of scans, ns). These parameters can be stored for further use (wpar dept45 all). Process the spectrum as described above.

The same procedure should be followed to record a DEPT-135 spectrum: read the predefined parameter set of DEPT-45 experiment (rpar dept45 all), change the pulse program (pulprog=dept135) and start the acquisition (check for the number of scans, ns). These parameters can be stored for further use (wpar dept135 all). On the other hand, the acquisition and processing steps can be performed in automation mode by typing xaua au_zg135 and xaup proc_cpd135, respectively.

Otherwhise, a single parameter set can be defined (wpar dept all) for recording any DEPT experiment. In such case, the pulse program is set to dept and the user must define the corresponding p0 pulse (45, 90 or 135 degree ¹³C observe pulse) according to the carbon multiplicity selection.

    1D 1H-decoupled carbon-13 spectrum