Water-Protein Interaction
An effective
selective water excitation scheme
is a key feature when NMR experiments are designed to study
water-protein interactions
(95JB306). Several approaches have been proposed:
A SPFGE block (
95JMRB334-109
,
96JMRB282-112
, although this scheme is very sensitive to diffusion and relaxation effects.
Use of slaved pulses (
99JMR107-138
).
The WEX filter (
94JACS11982
).
Differences in diffusion properties between individual water molecules and the protein. For instance, the HYDRA experiment (
96JACS11629
) or the DRYCLEAN scheme (
90JMR18-87
and
93JACS8907
).
Radiation damping effects can be minimized using a Q-switched probe (
95JMRB192-107
and
95JMRB218-109
) or using bipolar gradients between the succesive points of a shaped pulse (
96JB87
and
99JB73
). radiation damping has been also used to propose other water excitation schemes (
95JB420
)
A MS excitation scheme (
99JB73
)
A quadrature-free Constant-time approach (
99JB189
)
Water-Protein Interaction