Reduced Dimensionality

Wuthrich et al. ( 93JACS9307 , 93JB127 , 02PNAS8009 and 04ANG782 ) proposed a projection technique that allows to design new modified triple-resonance multi-dimensional NMR experiments that provide the same connectivity information in spectra with reduced dimensionality. Main features:

  • n chemical shifts can be encoded in a n-1 dimensional spectrum
  • Larger values of t(max) can be chosen for the indirect dimensions
  • More extensive phase cycling is feasible within the same accumulation time
  • Smaller data sets facilitates data handling and processing.
  • Multiple quadrature detection is feasible (See GFT-NMR)
  • General overview and comparison of several fast-NMR methods: 03JB101-27 and 04CONC63

    Some 2D and 3D NMR experiments using the so-called reduced dimensionality approach are:

  • 2D HN(CO)CA, 2D H(N)COCA, 2D HN(COCA)H, 2D HN(CA)CO, and 2D HN(CA)H experiments ( 94JMRB77-105)
  • 2D H[NCO] experiment ( 94JMRB77-105 and 94JB325 )
  • 2D H[NCA] experiment ( 93JACS9307 , 94JMRB77-105, and 94JB325 )
  • 3D HA[CAN]NH experiment ( 93JB127 )
  • 3D [HAHB-CACB](CO)NH experiment ( 94JMRB188-105, 96JACS8146, and 98JB387). Here, the information of 3D CBCA(CO)NH and 3D HBHA(CO)NH experiments is mixed in a single experiment .
  • 3D HMQC(13C)-NOESY-HMQC(15N) and 3D HSQC(15N)-NOESY-HMQC(15N) experiments ( 95JMRB238-109 )
  • 3D HN[COCA] ( 95JMRB197-108, 95JB202, 98JB387) and 3D [CO]HNN[CA] or COHNCA ( 95JMRB197-108 and 02JMR155-156 ) experiments.
  • 3D HN[CAHA] experiment ( 96JACS8146 and 98JB387)
  • 3D [HCA][CO(CA)N]NH experiment ( 95JB189) in which the chemical shift information of four different nuclei is monitored in only two indirect dimensions.
  • 2D [HC](C)H-COSY experiment ( 97JMR228-128 )
  • 3D [HN](COCA)NH experiment ( 97JB94 )
  • 2D HN(CO)CACB, HN(COCA)CACB, HN(CO)CAHA and HN(COCA)CAHA experiments ( 02JMR262-156 ) in which three different frequencies can be encoded in a single time domain.
  • 3D HNN[CAHA] and HNN(CO)[CAHA] in which CA and HA evolve simultaneously ( 02JB41-24 )
  • 3D HNN[COCA] and HNN[CACO] in which CA and CO are observed simultaneously ( 02JB41-24 )
  • Application:
  • Solid-State methods using reduced dimensionality ( 01JMR303-152 )
  • PWI Domain from SRm160 using 3D HNN[CAHA], 3D [HACA](CO)NHN, and 3D [HACA,HBCB](CO)NHN experiments ( 02JB299-22 )
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