G-Matrix Fourier Transform (GFT-NMR)

GFT-NMR ( 03JACS1385 and 04JB117-28) is an extension of the Reduced dimensionality NMR approach to accelerate acquisition of multi-dimensional NMR data. A smaller spectral width can be used in GFT-NMR compared to RD-NMR, by simultaneous acqusition of cosine- and sine-modulated components for each projection dimension, therevy allowing positive and negative frequencies to be separated. Thus, multiple quadrature detection is feasible ( 03JB157-26). Applications:

  • General overview and comparison of several fast-NMR methods: 03JB101-27 and 04CONC63 .
  • G2FT experiments in which two G-matrix transformations are applied in a (5,2)D H[NCOCACB] experiments ( 05JACS4554 )
  • Examples of (3,2)D GFT experiments have been reported:
  • 2D H[NCA], 2D H[N](CO)[CA], and 2D [HACAN]H experiments using multiple quadrature detection ( 03JB157-26) 04JB467-29
  • A suite of 2D 1H-15N-X correlation experiments: HNCA, HN(CA)CB, HN(CA)HA, HN(CO)CA, HN(COCA)CB, HN(COCA)HA. A related 2D J-HNCO is proposed to measure 1J(NH) and 2J(HN-CO) coupling constants from the same experiment. ( 03JB57-27).
  • 4D and 5D spectral information and analysis protocol for high-throughput protein structure determination ( 05PNAS10487 )
  • GFT applied to 4D and 5D-type experiments for a NOESY-based structure determination protocol ( 05JACS9085). Some experiments described are
  • RD version of the 4D HNCACB: (4,3)D HN[Cab,Ca]
  • RD version of the 4D CBCA(CO)NH: (4,3)D [Cab,Ca](CO)NH
  • RD version of the 5D HACACONH: (5,2)D [HACACON]H
  • RD version of the 4D HCCH: (4,3)D [HC]CH
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