Isotope-edited NMR experiments used to study 15N,13C-labeled protein and unlabeled ligand complexes:
 
 
NMR Experiment
Solvent
NOE contact
Contact type
3D 15N-edited NOESY
(3D NOESY- 1H-15N-HSQC 
or
3D NOESY- 1H-15N-HMQC)
H2O
Intramolecular protein 
Intermolecular protein-ligand
15N-1H to 1H
3D 13C-edited NOESY
(3D NOESY-1H-13C-HSQC 
or 3D NOESY-1H-13C-HMQC)
D2O
Intramolecular protein 
Intermolecular protein-ligand
13C-1H to 1H
4D 13C/13C-edited NOESY
(4D 1H-13C- HMQC-NOESY-1H-13C-HMQC)
D2O

Intramolecular protein
13C-1H to 1H-13C
4D 15N/13C-edited NOESY
4D 15N/15N-edited NOESY
(4D 1H-X-HMQC- NOESY-1H-X-HMQC)
H2O

Intramolecular protein
15N-1H to 1H-13C

15N-1H to 1H-15N
3D 15N/15N-edited NOESY
(3D 1H-15N-HMQC- NOESY-1H-15N-HMQC)
H2O

Intramolecular protein
15N-1H to 1H-15N
2D 13C(w1)/13C(w2)-filtered NOESY
(2D X-filtered NOESY)
D2O

Intramolecular ligand
12C-1H to 1H-12C
2D 13C(w1)/15N(w2)-filtered NOESY
2D 15N(w1)/15N(w2)-filtered NOESY
2D 15N(w1)/13C(w2)-filtered NOESY
(2D X-filtered NOESY)
H2O

Intramolecular ligand
12C-1H to 1H-14N
14N-1H to 1H-14N
14N-1H to 1H-12C
3D 15N(w1)-edited/15N(w3)-filtered NOESY
3D 15N(w1)-edited/13C(w3)-filtered NOESY
3D 13C(w1)-edited/15N(w3)-filtered NOESY
(3D 1H-X-HMQC- NOESY-1H-X-HMQC)
H2O

Intermolecular protein-ligand
15N-1H to 1H-14N
15N-1H to 1H-12C
13C-1H to 1H-14N
3D 13C(w1)-edited/13C(w3)-filtered NOESY
(3D 1H-13C-HMQC- NOESY-1H-13C-HMQC)
D2O

Intermolecular protein-ligand
13C-1H to 1H-12C