A series of related applications on proteins have been reported:
- Determination of the molecular aggregation state and solution properties of proteins ( 95JACS7566 , 97JB1-10 , 97BIO3383 , 97JMR468-124 , 95JB321-6 , and peptides ( 00JB109 ). Lysozyme has been used as a standard ( 99JACS11503 and , 98BBA311 , 00JACS5916 , 01BJ1585) but other proteins have been also studied such as Pichia farinosa killer toxin ( 99JB113-13 ).
It has been demonstrated that the dimerization of a protein reduces the diffusion coeficient about 29% (Dmonomer/Ddimer=1.4).
Ubiquitin (8.6 kDa): 1.49*10-6 cm2/s (monomeric)
MCP-1 (8.7 kDa): 1.08*10-6 cm2/s (dimeric)
Lysozyme (14.5 kDa): 1.08*10-6 cm2/s (monomeric)
Interleukin-10 (18.6 kDa): 0.82*10-6 cm2/s (dimeric)
- Protein folding ( 97JB199 , 00JACS5887 , 00BIO7910 , 02JACS7156 )
- Study of the interaction between protein and surfactants ( 95JPC828 )
- Quantitation of chemical exchange rates ( 97JB136 ) and determination of the relative NH lifetimes ( 99JB25 )
- Chemical exchange effects and measurement of exchange rates ( 93JMRA214-102 ).
- Practical aspects of the measurement of the diffusion of proteins in aqueous solution has been reported ( 98JMR379-133 ).
- Conformational analysis of flexible peptides ( 01FRE308 )
- Diffusion studies on several anisotropic media (01JACS12343 )
- Distribution of molecular size within an unfolded state ensemble (02JMB101 )
- Characterization of phospholipid mixed micelles (04JB299-29 )