Amino Acids: Thryptophan

TRYPTOPHAN TRP, W

CHEMICAL STRUCTURE

CHEMICAL SHIFTS

Standard chemical shifts of random extended chain structures:

¹H^a	¹³C^a	¹⁵N^b
NH: 8.09 HA: 4.70 HB: 3.32, 3.19 H2: 7.24 H4: 7.65 H5: 7.17 H6: 7.24 H7: 7.50 NH: 10.22	CO: 176.7 CA: 56.7 CB: 27.4 C1: C2: 126.0 C3: 108.4 C4: 120.3 C5: 122.9 C6: 119.3 C7: 112.8 C8: 137.3	N: 120.5

a) from 86BOOK1: b) from 91JMB311-222

More info in:

¹H chemical shifts of amino acids

¹³C chemical shifts of amino acids

Average ¹HA, ¹HN and ¹⁵N chemical shifts in random coil

IDENTIFICATION

Easy spin system identification from:

HA-HB cross-peak in a COSY-DQF experiment.

Typical AMX pattern in a TOCSY experiment.

SECONDARY STRUCTURE

Standard average chemical shift values used in determination of secondary structure by means of the CSI method:
HA: 4.70
CA: 57.6
CO: 175.6
Thus:
          HELIX           B STRAND          COIL
             CA      CO       CA      CO           CA      CO
            59.0    180.2     55.2    -      56.4    173.6
More info in: ¹³CA and ¹³CO chemical shifts according to secondary structure.

OBSERVATIONS

RELATED TOPICS