Amino Acids: Lysine

LYSINE LYS, K

CHEMICAL STRUCTURE

CHEMICAL SHIFTS

Standard chemical shifts of random extended chain structures:

¹H^a	¹³C^a	¹⁵N^b
NH: 8.41 HA: 4.36 HB: 1.85, 1.76 HG: 1.45 HD: 1.70 HE: 3.02 NH₃⁺: 7.52	CO: 174.7 CA: 54.4 CB: 27.5 CG: 23.1 CD: 31.8 CE: 40.5	N: 121.5

a) from 86BOOK1: b) from 91JMB311-222

More info in:

¹H chemical shifts of amino acids

¹³C chemical shifts of amino acids

Average ¹HA, ¹HN and ¹⁵N chemical shifts in random coil

IDENTIFICATION

Easy spin system identification from:

HA-HB cross-peak in a COSY-DQF experiment.

Typical pattern in a TOCSY experiment.

SECONDARY STRUCTURE

Standard average chemical shift values used in determination of secondary structure by means of the CSI method:
HA: 4.36
CA: 56.5
CO: 176.5
Thus:
          HELIX           B STRAND          COIL
             CA      CO       CA      CO           CA      CO
            59.3    178.4     54.8    175.6      56.6    176.5
More info in: ¹³CA and ¹³CO chemical shifts according to secondary structure.

OBSERVATIONS

RELATED TOPICS