Amino Acids: Glutamic Acid

GLUTAMIC ACID GLU, E

CHEMICAL STRUCTURE

CHEMICAL SHIFTS

Standard chemical shifts of random extended chain structures:

¹H^a	¹³C^a	¹⁵N^b
NH: 8.37 HA: 4.29 HB: 2.09, 1.97 HG: 2.31, 2.28	CO: 174.8 CA: 54.9 CB: 28.9 CG: 34.6 CD: 182.8	N: 121.3

a) from 86BOOK1: b) from 91JMB311-222

More info in:

¹H chemical shifts of amino acids

¹³C chemical shifts of amino acids

Average ¹HA, ¹HN and ¹⁵N chemical shifts in random coil

IDENTIFICATION

Easy spin system identification from:

HA-HB cross-peak in a COSY-DQF experiment.

Typical pattern in a TOCSY experiment.

SECONDARY STRUCTURE

Standard average chemical shift values used in determination of secondary structure by means of the CSI method:
HA: 4.29
CA: 56.5
CO: 176.6
Thus:
          HELIX           B STRAND          COIL
             CA      CO       CA      CO           CA      CO
            59.2    178.5     54.6    175.0      56.4    176.6
More info in: ¹³CA and ¹³CO chemical shifts according to secondary structure.

OBSERVATIONS

RELATED TOPICS