Amino Acids: Glutamine

GLUTAMINE GLN, Q

CHEMICAL STRUCTURE

CHEMICAL SHIFTS

Standard chemical shifts of random extended chain structures:

¹H^a	¹³C^a	¹⁵N^b
NH: 8.41 HA: 4.37 HB: 2.13, 2.01 HG: 2.38 NH2(E): 7.59, 6.87	CO: 174.0 CA: 54.1 CB: 28.1 CG: 32.2 CD: 179.0	N: 120.3

a) from 86BOOK1: b) from 91JMB311-222

More info in:

¹H chemical shifts of amino acids

¹³C chemical shifts of amino acids

Average ¹HA, ¹HN and ¹⁵N chemical shifts in random coil

IDENTIFICATION

Easy spin system identification from:

HA-HB cross-peak in a COSY-DQF experiment.

Typical pattern in a TOCSY experiment.

SECONDARY STRUCTURE

Standard average chemical shift values used in determination of secondary structure by means of the CSI method:
HA: 4.37
CA: 56.0
CO: 176.6
Thus:
          HELIX           B STRAND          COIL
             CA      CO       CA      CO           CA      CO
            58.7    178.1     54.0    174.6      55.8    175.6
More info in: ¹³CA and ¹³CO chemical shifts according to secondary structure.

OBSERVATIONS

RELATED TOPICS