The selective ge-1D HMQC-COSY experiment permit to obtain high-quality 1D HMQC-COSY spectra from which 1H-1H J-correlation can be elucidated starting from a selected carbon. Thus, in the first part of this experiment magnetization is transferred from the selected carbon to the directly-bonded protons via an HSQC block. After this, the in-phase magnetization of these protons is allowed to evolve by a conventional COSY block under the effect of homonuclear J(HH) coupling. The same principles described here are also applied to the selective ge-1D HSQC-COSY experiment.

Easy implementation on AVANCE spectrometers equipped with pulsed field gradients, selective excitation using shaped pulses and inverse probehead.

The basic pulse sequence of the selective ge-1D HMQC-COSY experiment is exactly the same as the conventional ge-2D HMQC-COSY pulse train in which the following modifications have been included ( 95JMRA32-114 ):

• One of the two 90º 13C pulses is made selective on a specific heteronucleus. In order to improve the selectivity, proton decoupling is simultaneously applied during the long selective 13C pulse.
• The variable evolution period of the 2D version is fixed to a  minimum delay (3 microseconds).
• Gradients are usually applied for coherence selection purposes in natural abundance samples to obtain efficient suppression of 1H-12C magnetization.

The selective ge-1D HMQC-COSY experiment can be run with minor changes from a predefined parameter set. The HMQC block is optimized as discussed in the selective ge-1D HMQC experiment and the COSY delay is optimized as usual.Important parameters to consider are:

Selectivity of the selective excitation 13C pulse: the user must define the offset, the shape, the duration and the power level needed for a defined excitation profile.

Optimization of the J-coupling delay in the HMQC block as a function of 1/(2*J(CH)), in order to get in-phase magnetization of the directly-bonded protons.

Optimization of the J-coupling delay in the COSY block as a function of 1/(2*J(HH)), in order to get antiphase magnetization of all protons J-coupled with the directly-bonded proton.

The selective ge-1D HMQC-COSY experiment affords a simple 13C-edited COSY spectrum in which the directly-bonded protons to the selected carbon appear as large doublet due to 1JCH and the protons J-coupled with them show the commonly anti-phase pattern of COSY signals. The use of gradients allows to obtain a clean, artefact-free spectrum in a short time in which perfect suppression of undesired 1H-12C magnetization is achieved with a single two-step phase cycle (The selective 90º carbon pulse and the receiver are usually inverted on alternated scans).