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The 3D HN(CA)CB[CO]-E.COSY experiment allows to measure 3J(CO-CB) coupling constants in 13C,15N-labeled proteins dissolved in H2O from E.COSY multiplet patterns.. REQUIREMENTS
Implementation on AVANCE spectrometers equipped with a third channel and pulsed field gradients (PFGs).VERSIONS
The original pulse sequence ( 99JB263 ):EXPERIMENTAL DETAILS
SPECTRA
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